innuPREP Blood DNA Midi Kit
Features
- Extremely simple tool for isolating genomic DNA from 0.5 ml to 2 ml whole blood samples
- Excellent purity and yields of up to 50 µg
- Processing in mini-Spin Filter format despite large starting volumes
- Patented, optimized extraction chemistry
Product Info
The innuPREP Blood DNA MIidi Kit has been specially designed as an effective tool for isolating genomic DNA from 0.5 ml to 2 ml samples of whole blood. The kit combines three steps: erythrocytes are first selectively digested, after which nucleated blood cells are pelleted and then lysed. After a precipitation step to selectively remove proteins, the DNA is bound to the surface of a Spin Filter membrane and then eluted. The extraction process involves the use of standard bench-top centrifuges and operates on a minicolumn scale. Combining a highly stringent Lysis Buffer with a novel Binding Buffer makes it possible to achieve unique yields of highly pure DNA.
Order Info
Order number | Quantity |
|---|---|
845-KS-1030010 | 10 reactions |
845-KS-1030050 | 50 reactions |
Specifications
Starting material:
- 0.5 to 2 ml samples of whole blood
- Fresh or frozen blood
- Stabilizers: EDTA or citrate
Extraction time:
Approx. 30 – 40 minutes, including lysis
Binding capacity:
Column binding capacity: > 100 µg gDNA
Average yield:
- Depends on the sample and the volumes used
- Approx. 10 – 50 µg gDNA
Average purity:
1.7 – 2.0
Downloads
- Brochure Product Guide Extraction .pdf | 1.67 MB
- Manual innuPREP Blood DNA Midi Kit .pdf | 2.44 MB
- MSDS innuPREP Blood DNA Midi Kit .pdf | 908.08 kB
(e.g. Manual, Short Manual)
