innuPREP Blood DNA Master Kit

Features

• Uncomplicated method for isolating genomic DNA from 0.5 ml to 5 ml whole blood samples
• Extremely high yields of up to 100 µg gDNA
• Extraction process using mini-Spin Filter, despite large starting volumes
• Optimized PLP Lysis Tubes reduce the pipetting work involved and make the process considerably less timeconsuming

Product Info

The innuPREP Blood DNA Master Kit is used for extracting genomic DNA from 0.5 to 5 ml whole blood samples. The basis for the test is an optimum combination of three steps: selective digestion of erythrocytes, along with pelleting and subsequent lysing of nucleated blood cells. The lysis step is performed in what are known as PLP Tubes, which already contain all of the reagents required as well as the proteolytic enzymes, all in a stable form. This is followed by three additional steps: selective removal of proteins via precipitation, binding of the gDNA to a Spin Filter membrane, and the final elution. Standard bench-top centrifuges for mini-Spin Filters can be used despite the starting quantity in the master format.

Order Info

Specifications

Starting material:

• 0.5 – 5 ml samples of whole blood
• Fresh or frozen blood
• Stabilizers: EDTA or citrate

Extraction time:

Approx. 30 – 40 minutes, including lysis

Binding capacity:

Column binding capacity: > 100 µg gDNA

Average yield:

• Depends on the sample and the starting volumes used
• Approx. 10 – 100 µg gDNA

Average purity:

1.7 – 2.0

Downloads

• Brochure Product Guide Extraction
  .pdf | 1.67 MB
• Manual innuPREP Blood DNA Master Kit
  .pdf | 1.55 MB
• MSDS innuPREP Blood DNA Master Kit
  .pdf | 918.03 kB

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