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Configuration examples for MALDI-TOF-MS sample preparation

Standard MALDI sample preparation

Capacity

  • 1x MALDI plate
  • 1-4x sample plate(s)

Throughput

  • < 10 min to prepare a MALDI target plate

Start situation

  • 1x Matrix reservoir
  • 1x Tip wash station wash buffer
  • 1x MALDI plate
  • 1-4x source plate(s)

Extended standard MALDI sample preparation

Including plate storage

Capacity

  • CyBio QuadStack M: 90 MALDI plates or 30 source plates (1536) and 30 MALDI plates or 40 source plates (384) and 10 MALDI plates

Throughput

  • < 10 min to prepare a MALDI target plate

Start situation

  • 1x Matrix reservoir
  • 1x TWS wash buffer
  • 1-30x MALDI plates
  • 1-40x source plate(s)

Fully automated MALDI-TOF sample preparation

Including storage for source and MALDI plates

Capacity

  • Plate storage - Hotel: 10 x 21 = 210 sample plates
  • CyBio QuadStack M: 90 MALDI plates

Throughput

  • < 10 min to prepare a MALDI target plate

Start situation

  • 1x Matrix reservoir
  • 1x TWS wash buffer
  • 1-90 x MALDI plates

1-210 x source plate(s)

CB-Vario deck layout

  1. MALDI plate
  2. Source plate
  3. Matrix reservoir
  4. De-ionisation
  5. Tip touch station
  6. Tip wash

Extended fully automated MALDI-TOF sample preparation

Including plate store, sealer, peeler, centrifuge

Capacity per batch

  • 90 MALDI target plates (CyBio QuadStack (M) 3 racks) and 180 (CyBio QuadStack (L) 3 racks) source plates

Throughput

  • < 10 min to prepare a MALDI target plate

Start situation

  • 1x Matrix reservoir
  • 1x TWS wash buffer
  • 1-90 x MALDI plates
  • 1-180 x source plate(s)

MALDI-TOF reader plate delivery set up

Including CyBio QuadStack M

Capacity

  • 1 - 90 plates

Throughput

  • 10 s for plate handling

Start situation

  • MALDI plates sorted in the CyBio QuadStack M

Ausgangssituation

MALDI-Platten sortiert im Regal

Including random accesses plate storage

Capacity

  • 1 - 110 plates

Throughput

  • 10 s for plate handling

Start situation

MALDI plates sorted in the shelf

Components

Analytik Jena devices

Component

Description

CyBio® Well vario Pipettor

Base station without pipetting head; combine with a circular transport system and a pipetting head to form a functional system.

10 position circular transport system

Circular transport system with base frame and rotary table with10 positions

CyBio® Well vario Pipetting head with1536/8 μl or 384/25 μl Tip Magazine

CyBio® Well vario Pipetting Head for 384- or 1536-well simultaneous liquid handling and Fixed Tip Magazine 1536/1 µl or 384/25 µl Ceramic with individually changeable non-disposable  ceramic tips

CyBio® Well vario Barcode reader NLV 3101

For reading barcode labels on the short side of microplates on linear transport system

Pump module

Pump module with 2 pumps and power supply

Tip wash station 1536

Complete tip wash station 1536 set

MALDI sample adapter 1536

For optimal dry transfer of small volumes. Optimized for Bruker MALDI sample plate/MALDI microplates with 127.7 mm x 85.4 mm footprint

Microplate adapter 1536

For use with 1536-well microplates with CyBio® Well vario

Reservoir 1536

Optimized for applications with CyBio® Well vario Pipetting Head

1536 with refillable option on request

Active dryer

Park deck and vacuum chamber to reduce the drying time for MALDI sample plate

CyBio® QuadStack M

Medium sized stacker with 30 SW plates capacity per stack, MALDI sample plate storage

 

 

Third party example devices

MS reader

High speed mass spectrometer

Robotic arm

4 Axis Scara robotic arm with 750 mm vertical stroke, servo gripper

Incubator

Storage of assay plates (optional with lid) with a capacity for 210 shallow well plates

Peeler

Fully automated seal removal in wide variety of environments

Sealer

Thermal microplate sealer, accommodates microplates as tall as 63.5 mm, sealing temperature 30 to 200°C, cycle time ~ 8 seconds per plate

Centrifuge

Micro plate centrifuge for up to 5000 x g,  imbalance tolerant: up to 100 g weight difference between buckets, 2-bucket rotor, maximum plate height: 61 mm

 Hotel

Carousel hotel package, 10 place, 220 capacity, package with 10 extended-height random access hotels

 

 

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