innuDETECT Halal & Halal Multiplex Assay

In multicultural societies food industry increasingly faces the challenge of food stuff production complying with religious laws. This includes determination of origin of gelatin in gummy bears, type of meat in minced meat or even the identification of pork traces in rice. For identification of closely related animal species and, hence, for determination of a food product being ’halal’ Real-Time PCR has been proven as reliable, precise and fast approach. The innuDETECT Halal Multiplex Assay is a novel developed TaqMan^®-based screening system enabling the highly specific multiplex analysis of donkey/horse DNA (FAM channel) and pork DNA (HEX channel) in samples of diverse starting materials. The lyophilized kit includes an additional Internal Control (IC) to be used as extraction and/or amplification control (Cy5 channel). The innuDETECT Halal Assay realizes detection of pork, horse and donkey DNA in three separate reactions in order to reach the maximum of sensitivity from up to 10 copies/PCR in the multiplex assay to up to 5 copies/PCR in the 3-Tube Duplex. The Real-Time assays of Analytik Jena offer a precise answer to the crucial question of muslims: „Is what we are eating »halal«?“.