Biometra TAdvanced

Features

Biometra TAdvanced – the high-performance thermal cycler for DNA amplification by PCR

Twelve different sample blocks: including a 96-well silver block as high-end variant
Powerful control electronics: Now even an aluminum block reaches superior heating and cooling rates as usually only achieved in instruments with silver block
Fastest Ramping, Highest Accuracy and Intelligent Block/Well Control (RAC): high temperature uniformity and maximum reproducibility, no over- or undershooting of the programmed target temperature
Optional with gradient: simple optimization of new primer pairs
Linear Gradient Tool (LGT): specific adjustment of the gradient temperature profile
High Performance Smart Lid Technology (HPSL): fast heating, constant contact pressure and easy use
Quick Block Exchange System: easy, fast exchange of sample blocks
Whisper Quiet Technology: particularly quiet operation with a maximum noise level of 45 dB
Intuitive software: graphical programming and Easy Spreasheet Programming
Multi Step Programming: easy creation of PCR protocols during programming without the need to toggle between different windows
Advanced User Management (AUM): flexible user management with individual assignment of user rights for single users
User Specific Quick Start: no tedious searching of already used PCR programs due to the combination of Quick Start and Program Preview
Extended Self Test: automatic system diagnosis and documentation of PCR runs
Comfortable 7 inch color touch display
USB and Ethernet port
Protocol Wizard: Automatic creation of PCR protocols with few inputs

Biometra TAdvanced - No Compromises in Technology

Product Info

The Biometra thermal cycler for highest demands

High-Speed Thermal Block
The Biometra TAdvanced Thermal Cycler is available with different block formats. The Quick Block Exchange technology allows an easy exchange of block modules in a few seconds. All blocks are made of aluminum and offer high ramp rates for fast protocol running times. Additionally, to achieve ultimate performance, a 96 well block made of silver is available. Due to its excellent heat conductivity, silver equilibrates extremely quickly, thus providing maximum speed and temperature uniformity. To protect the valuable silver blocks against corrosion, the block surfaces are gold plated. By the rubber sealing at the lid an encapsulated space is formed when the lid is closed. The closed room serves to improve the temperature uniformity of the sample block and avoids formation of condensed water at the final PCR cooling step. Furthermore all blocks are perfectly sealed to prevent condensed water from penetrating the Peltier elements underneath the sample block and other parts of the electronics. The Peltier elements are protected by the perfect sealings. This guarantees a longer intrument lifetime.

High-Performance Smart Lid
The Biometra TAdvanced heated lid has been optimized to heat twice as fast as with older models and thus helps to shorten protocol run times. Moreover the heated lid serves for two additional essential functions: It prevents the formation of condensed water at the reaction tube portion located above the block surface level and it ensures reliable contact between samples and thermal block by applying constant pressure. By the integrated clutch mechanism the pressure applied to the reaction tubes is always same regardless of the plastic ware height. The combination of the heated lid shape and the reliable contact pressure ensure an even temperature distribution between samples thus significantly improves the temperature uniformity.
With one press on the front button, the lid gently swings open and arrests in its end position. By this mechanism the lid cannot fall down and the heated lid is out of the radius of action when the user is inserting tubes or plates. Thus the risk of injury by violent pressure or the risk of burn by hot surfaces is prevented.

State of the Art Touchscreen (Display)
The Biometra TAdvanced Thermal Cycler features a 7`` state of the art color touchscreen user interface. The touchscreen is built-in at a flat angle to ensure reflection-free viewing and ergonomic programming. For programming of PCR protocols, the software incorporates Biometras proven spreadsheet philosophy and additionally a graphical programming mode. One touch leads from the spreadsheet to the alternative graphical programming mode. This makes the creation of new or editing existing of programs fast and easy.

User specific quick start function
Many other thermal cyclers offer a so called “latest program list”. In the latest program list a certain number of PCR programs is collected and offered for quick start. The list is typically ordered only by date and the user has to search for the desired program. In contrast the Biometra TAdvanced software creates a user specific list and only the latest programs started by the currently logged-in user are offered for quick start. Even a lab member does not use the instrument for a longer time the information on the latest programs started by this user will not get lost.
For maximum convenience the Biometra TAdvanced software features additionally features the program preview. Before start of a program the protocol steps are summarized in a clearly arranged table by the program preview tool. The program preview therefore provides a comprehensive overview on the protocol structure without the need to access programming screens. The program preview is also available before quick start of programs.

Linear Gradient Tool for maximum convenience
For optimizing new primer pairs in a single run the Biometra TAdvanced optionally can be equipped with gradient function. Finding the best primer annealing temperature is crucial for the specificity and efficiency of PCR reactions. Often optimization of experiments is conducted only limited leading to unspecific byproducts or reduced PCR sensitivity. By using the gradient function new primer pairs with unknown annealing temperatures can be tested quickly and optimized in very short time.
For maximum convenience the Biometra TAdvanced offers the Linear Gradient Tool. For most other instruments the temperature difference from row to row or from column to column in a gradient step varies across the sample block. The Biometra TAdvanced Linear Gradient Tool offers to program gradient steps with a defined temperature increment between the columns or rows. The Linear Gradient Tool option allows the comfortable creation of gradient steps and the best annealing temperature can be easily transformed to a non-gradient protocol.

Security for regulated enviroments
The software can manage up to 30 user accounts. By default three different user-levels are available: Administrator, user with generic rights and user with limited rights. However, in a comfortable menu the administrator can configure the settings for each user individually by activating or inactivating single rights. By the user administration tool in combination with the password protection of user accounts access to the instrument can be limited to authorized persons and unwanted modifications to the system settings and PCR protocols prevented.
With each system start the Biometra TAdvanced performs an initial self-test and additionally the software offers an extended self-test that can be initiated by the user. The results of the extended self-test becomes are summarized in a protocol and are stored by the instrument. Additionally to the self-test functions the instruments creates and stores run-logfiles for each single run. For long-term storage run-logfiles and extended self-test protocols can be exported as proprietary file format.

Fastest Ramping, highest Accuracy, Block/Well Control:
Superior sample block temperature control
Whisper Quiet: Low noise emission of max. 45 dB
High Performance Smart Lid: Defined pressure control for
highly reproducible results
Quick Block Exchange: Easy exchange of block modules thanks
to optional QBE technology
Linear Gradient Tool: For easy gradient programming using the
primer annealing temperature
Protocol Wizard: Simple protocol creation by just a few inputs
Advanced User Management: Individual rights settings for each
user

Technical Data

Instrument	Biometra TAdvanced 96/96 G	Biometra TAdvanced 96 S/96 SG
Capacity	96 x tubes 0.2 ml 96 well microplates 12 x strips 8 well	96 x tubes 0.2 ml 96 well microplates
Block	Aluminum	Silver
Block coating	Special Alloy	Gold
Block Exchange	Quick-X-Change	Quick-X-Change
Block Exchange time	less than 10s	less than 10s
Max. heating rate*	6,0 °C/sec	8.0 °C/sec.
Max. cooling rate*	4,0 °C/sec	5.5 °C/sec.
Avg. Heating Rate*	5,5 °C/sec	7.0 °C/sec.
Avg. Cooling Rate*	3,5 °C/sec	4.5 °C/sec.
Max./min. Gradient span¹	30 °C/0.1 °C	40 °C/0.1 °C
Temperature Uniformity	95 °C ± 0.60 °C after 15 s 70 °C ± 0.30 °C after 15 s 55 °C ± 0.20 °C after 15 s	95 °C ± 0.50 °C after 15 s 70 °C ± 0.25 °C after 15 s 55 °C ± 0.15 °C after 15 s
Gradient Temperature Range¹	20 °C to 99 °C	4 °C to 99 °C
Temperature Range	3 °C to 99 °C
Control Accuracy	+/- 0.1 °C
Software	User specific quick start of the last five programs, program preview prior to start, toggle between spreadsheet and graphical programming mode, Linear Gradient Tool¹, generate service info files (SINF),extended self-test, adjustable ramp rates, view gradient temperature graph¹, PC control via Ethernet, comprehensive user administration tool with individual rights settings, Protocol Wizard
Display	7‘‘ Color Touchscreen
Auto restart after power failure	Yes
High Performance Smart Lid (HPSL) Technology	Yes
Lid Temperature Range	30 °C to 110 °C
Power consumption	850 Watt
Power Supply	100, 115, 230 Volt, 50-60 Hz
Noise emission	Very low, max. 45 dB
Interfaces²	USB A, Ethernet
Working conditions	15 °C to 35 °C, 70 % air humidity, max. 2000 m NN
Dimensions (W x D x H)	277 mm x 457 mm x 264 mm
Dimensions (W x D x H) with lid opened	277 mm x 457 mm x 414 mm
Weight	15 kg
* measured inside the block ¹ Only for model type “G” featuring gradient function ² The network cable must be at least performance class Cat 5e and the cable configuration has to be STP

Instrument	Biometra TAdvanced 60/60 G	Biometra TAdvanced 384/384 G
Capacity	60 tubes 0.5 ml	384 well microplates
Block	Aluminum	Aluminum
Block coating	Special Alloy	Special Alloy
Block Exchange	Quick-X-Change	Quick-X-Change
Block Exchange time	less than 10s	less than 10s
Max. heating rate*	6.0 °C/sec	4.0 °C/sec
Max. cooling rate*	4.0 °C/sec	2.0 °C/sec
Avg. Heating Rate*	5.5 °C/sec	3.8 °C/sec
Avg. Cooling Rate*	3.0 °C/sec	1.7 °C/sec
Max./min. Gradient span¹	30 °C	24°C
Temperature Uniformity	95 °C ± 0.60 °C after 15s 70 °C ± 0.30 °C after 15s 55 °C ± 0.20 °C after 15s	95 °C ± 0.50 °C after 15s 70 °C ±0.25 °C after 15s 55 °C ± 0.15 °C after 15s
Gradient Temperature Range¹	20 °C to 99 °C	20 °C to 99 °C
Temperature Range	3 °C to 99 °C
Control Accuracy	+/- 0.1 °C
Software	User specific quick start of the last five programs, program preview prior to start, toggle between spreadsheet and graphical programming mode, Linear Gradient Tool¹, generate service info files (SINF),extended self-test, adjustable ramp rates, view gradient temperature graph¹, PC control via Ethernet, comprehensive user administration tool with individual rights settings, Protocol Wizard
Display	7‘‘ Color Touchscreen
Auto restart after power failure	Yes
High Performance Smart Lid (HPSL) Technology	Yes
Lid Temperature Range	30 °C to 110 °C
Power consumption	850 Watt
Power Supply	100, 115, 230 Volt, 50-60 Hz
Noise emission	Very low, max. 45 dB
Interfaces²	USB A, Ethernet
Working conditions	15 °C to 35 °C, 70 % air humidity, max. 2000 m NN
Dimensions (W x D x H)	277 mm x 457 mm x 264 mm
Dimensions (W x D x H) with lid opened	277 mm x 457 mm x 414 mm
Weight	15 kg
* measured inside the block ¹ Only for model type “G” featuring gradient function ² The network cable must be at least performance class Cat 5e and the cable configuration has to be STP

Instrument	Biometra TAdvanced Twin 48/48G	Biometra TAdvanced Twin 30	Biometra TAdvanced Twin combi
Capacity	2 x 48 x 0.2 ml tubes/ 2 x 48- well microtiter plate /2 x 6 x 8-well strips, 0.2 ml	2 x 30 x 0.5 ml tubes	2 x 48 x 0.2 ml tubes/ 2 x 6 x 8-well strips, 0.2 ml/2 x 48-well microtiter plate/ 2 x 18 x 0.5 ml tube
Block	Aluminum, special alloy	Aluminum, special alloy	Aluminum, special alloy
Max. heating rate¹	5.2 °C/s	4.2 °C/s	3.1 °C/s
Max./min. gradient²	20 °C/0.1 °C in one block of the twin-block	-	-
Gradient temperature range²	20 °C to 99 °C in one block of the twin-block
Temperature uniformity at 55 °C after 15 s	± 0.20 °C	± 0.20 °C	± 0.20 °C
Temperature range	3 °C to 99 °C	3 °C to 99 °C	3 °C to 99 °C
Control accuracy	± 0.1 °C	± 0.1 °C	± 0.1 °C
Lid temperature range	30 °C to 110 °C	30 °C to 110 °C	30 °C to 110 °C
Program memory	350 programs in up to 90 user directories	350 programs in up to 90 user directories	350 programs in up to 90 user directories
Dimensions (W x D x H, mm)	277 x 457 x 264	277 x 457 x 264	277 x 457 x 264
G = with gradient function 1 measured within sample block 2 applies only to cycler models with gradient function For more technical details please refer to the corresponding Technical Data sheets.

Order Info

Instruments
Order number	Description	Capacity
846-x-070-211	Biometra TAdvanced 96	96 well 0.2 ml tubes, plates, strips
846-x-070-201	Biometra TAdvanced 96 G	96 well 0.2 ml tubes, plates, strips
846-x-070-241	Biometra TAdvanced 96 SG	96 well 0.2 ml tubes, plates, strips
846-x-070-251	Biometra TAdvanced 96 S	96 well 0.2 ml tubes, plates, strips
846-x-070-210	Biometra TAdvanced 60	60 well 0.5 ml tubes
846-x-070-200	Biometra TAdvanced 60 G	60 well 0.5 ml tubes
846-x-070-224	Biometra TAdvanced 384	384 well 384 well plates
846-x-070-214	Biometra TAdvanced 384 G	384 well 384 well plates
846-x-070-212	Biometra TAdvanced Twin 48	2 x 48-well block (0.2 ml)
846-x-070-202	Biometra TAdvanced Twin 48 G	2 x 48-well block (0.2 ml) and gradient function in one block
846-x-070-213	Biometra TAdvanced Twin 30	2 x 30-well block (0.5 ml)
846-x-070-215	Biometra TAdvanced Twin combi	2 x 48-well (0.2 ml) and 2 x 18-well (0.5 ml) block
G = Gradient x = 2 for 230 V, 4 for 115 V, 5 for 100 V

Block modules and base unit
Order number	Description	Capacity
846-070-231	Biometra TAdvanced Block Module 96	96 well 0.2 ml tubes, plates, strips
846-070-221	Biometra TAdvanced Block Module 96 G	96 well 0.2 ml tubes, plates, strips
846-070-271	Biometra TAdvanced Block Module 96 S	96 well 0.2 ml tubes, plates, strips
846-070-261	Biometra TAdvanced Blockmodul 96 SG	96 well 0.2 ml tubes, plates, strips
846-070-230	Biometra TAdvanced Block Module 60	60 well 0.5 ml tubes
846-070-220	Biometra TAdvanced Block Module 60 G	60 well 0.5 ml tubes
846-070-234	Biometra TAdvanced Block Module 384	384 well plates
846-070-224	Biometra TAdvanced Block Module 384 G	384 well plates
846-070-232	Biometra TAdvanced Twin Block Module 48	2 x 48-well block (0.2 ml)
846-070-222	Biometra TAdvanced Twin Block Module 48 G	2 x 48-well block (0.2 ml) and gradient function in one block
846-070-233	Biometra TAdvanced Twin Block Module 30	2 x 30-well block (0.5 ml)
846-070-235	Biometra TAdvanced Twin Block Module combi	2 x 48-well (0.2 ml) and 2 x 18-well (0.5 ml) block
846-x-070-280	Biometra TAdvanced Base Unit	-

Downloads

Produktbroschüre Biometra Thermal Cycler Family
.pdf | 1.12 MB

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