innuTaq DNA Polymerase

Features

• Optimal for routine PCR
• Convenient and reliable amplification
• Extremely thermostable
• Best reproducible results

Product Info

Analytik Jena‘s innuTaq DNA Polymerase is a highly purified recombinant thermostable DNA polymerase that has been isolated from E.coli carrying a vector encoding the Thermus aquaticus DNA polymerase gene. The enzyme has 5’ → 3’ DNA polymerase activity. It exhibits high thermal stability in withstanding prolonged incubations at elevated temperatures (95 °C). It is recommended for use in routine PCR reactions.

Order Info

Specifications

Concentration:

5 U/µl

(One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into a polynucleotide fraction in 30 minutes at 70 °C.)

Enzyme storage buffer:

20 mM Tris-HCl pH 8.0; 100 mM KCl; 1 mM DTT; 0.1 mM EDTA; 0.5 % Tween 20; 0.5 % Nonidet P40; 50 % Glycerol

PCR buffer:

• 10× PCR Buffer with KCl; 100 mM Tris-HCl (pH 8.8 at 25 °C); 500 mM KCl; 0.8 % Nonidet P40
• 10× PCR Buffer with (NH₄)₂SO₄; 750 mM Tris-HCl (pH 8.8 at 25 °C); 200 mM (NH₄)₂SO₄; 0.1 % Tween 20

PS-Mg2+ solution:

MgCl₂ stock solution, 25 mM

Downloads

• Broschüre Product Guide Reagents/Consumables
  .pdf | 1.40 MB
• Manual innuTaq DNA Polymerase
  .pdf | 417.77 kB

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