PME free-circulating DNA Kit-IPC16
Features
- Automized extraction of free-circulating DNA
- Processing of starting sample volumes up to 10 ml
- Novel, patent pending technology: Polymer Mediated Enrichment (PME)
- Enrichment & extraction in approx. 76 minutes from 1 ml or approx. 1,5 h from 5 ml of serum or plasma and up to 10 ml from urine
- For usage of InnuPure® C16 touch and up to 16 samples in parallel
Product Info
Circulating cell-free DNA is a very interesting diagnostic target, but the amount of freecirculating DNA is usually very low and varies among different individuals. Further, these nucleic acids are present as short fragments, typically smaller than 1000 nt, making the efficient extraction process challenging. Because of the high sample volumes the protocols of commercially available kits are very labor-intensive as well as time consuming and need a lot of reagents.
The PME free-circulating DNA Kit-IPC16 is based on a new, patent-pending technology called PME – Polymer Mediated Enrichment. As first step cell-free DNA in the entire sample is captured by a polymer. Afterwards this complex is collected as a pellet by centrifugation. Subsequently the captured nucleic acid is dissolved in a special buffer thus reducing the sample volume in the following extraction significantly. Subsequently the extraction of free-circulating DNA from up to 16 samples in parallel takes place within InnuPure® C16 touch.
Order Info
Order number | Quantity |
|---|---|
845-IPS-6116016 | 16 reactions |
845-IPS-6116096 | 96 reactions |
845-IPP-6116016 | 16 reactions |
845-IPP-6116096 | 96 reactions |
IPS = Kit contains prefilled reagent strips for processing individual samples | |
Specifications
Starting material:
- Serum, plasma and urine
- Cell culture supernatants or mediums
- Other cell-free body fluids
- From up to 5 ml (serum and plasma) or 10 ml (urine)
Extraction time:
Enrichment (external):
- From 1 ml starting sample: approx. 10 minutes
- From 5 ml and 10 ml starting sample: approx 30 minutes
- InnuPure® C16 touch: approx. 66 minutes (1 ml) - 70 minutes (5 – 10 ml)
Downloads
- Product flyer PME .pdf | 491.17 kB
- Manual PME free circulating DNA Kit IPC16 .pdf | 1.28 MB
