innuPREP DNase I Kit
Features
- Efficient digestion of DNA traces in RNA samples
- High yields of high quality RNA
- Free of RNases and other interfering factors
- Ideal after finished RNA extraction
Product Info
innuPREP DNase I reliably and efficiently digests even the tiniest amounts of DNA that could potentially contaminate extracted RNA samples. After using a non-sequence-specific DNA endonuclease to cleave the DNA, the reaction is inactivated through the addition of the EDTA solution provided. Depending on the desired RNA purity level, a final ethanol precipitation is recommended in order to remove process-related impurities. All reagents are free of RNases and, as such, also contribute to the quality of the RNA. innuPREP DNase I is used for preparing DNA-free RNA, for breaking down the DNA template before transcription to cDNA, for DNase footprinting, and for Nick translation. The innuPREP DNase I Digest kit is recommended for removing DNA during column-based RNA isolation.
Order Info
Order number | Quantity |
|---|---|
845-KS-5210010 | 10.000 Kunitz units |
Specifications
Starting material:
RNA samples contaminated with DNA
Concentration:
DNase I, 1 KU/μL
Time of preparation:
30 min. for digestion, 10 minutes for inactivation
Quality of viral RNA:
Positive TaqMan® Real-Time PCR testing results
