innuPREP Mycobacteria DNA Kit

Features

  • Isolation of mycobacterial DNA from sputum, bronchoalveolar lavage or lymph node
  • Especially effective thanks to sample material pretreatment with N-acetyl cysteine
  • High-quality, ready-for-use DNA for all downstream applications

Product Info

The innuPREP Mycobacteria DNA Kit selectively extracts mycobacterial DNA from sputum, bronchoalveolar lavages or tissue samples (e. g. lymph nodes). The extraction process includes a step in which samples are pretreated with N-Acetyl cysteine followed by digestion with lysozyme and Proteinase K. After sample digestion, DNA is bound (under optimized binding conditions) to a Spin Filter membrane, washed and then desorbed from the filter membrane through the addition of a low-salt buffer. Special protocols adapted to different starting materials ensure maximum DNA yields and outstanding purity.

Order Info

Order number

Quantity

845-KS-6100010

10 reactions

845-KS-6100050

50 reactions

Specifications

Starting material:

  • Sputum samples, 0.2 – 5.0 ml
  • Bronchoalveolar lavages, up to 1.0 ml
  • Tissues (e. g., lymph nodes) ranging in size from 1.0 mg to no more than 10 mg

Extraction time:

Approx. 15 minutes after lysis

Downloads

Download on Request
(e.g. Manual, Short Manual)

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